Antibody Engineering

Fusion Antibodies offers a range of antibody engineering services including chimeric, scFv, Fab, F(ab')2, bi-specific and more!

We are experts at antibody engineering and can produce a range of custom antibodies

Fusion Antibodies offer a range of custom antibody configurations which are sure to meet your requirements. We can produce antibodies in a wide range of formats including scFv, Fab, chimerics and many more. We can also combine our antibody engineering services with our humanization services and express your humanized antibodies in an alternative format. There is no other company in the world that has the same experience in engineered antibody production. At Fusion Antibodies we combine 15+ years of antibody engineering experience with industry leading knowledge and expertise and are happy to discuss any collaboration on a new antibody engineering project.

Popular engineered antibody configurations

Fusion Antibodies offer a range of Antibody Engineering services. Some of our most popular antibody configurations are shown below but many more are possible. If you have an idea for an alternative antibody construct contact us today and we would be happy to disucss!

scFv antibody fragments

There are many advantages in using scFv’s, particularly in the fields of therapeutics and diagnostics. scFv’s offer several advantages over monoclonal antibodies as carriers of radionuclei and drugs to tumors, including greater tumour penetration due their small size, low kidney uptake, rapid blood clearance and a lower negative response by the human immune system.

For more information contact us today! Email or call UK +44 (0)28 9043 2800

Fab and F(ab')2 antibody fragments

Fab fragments differ from scFv’s as they contain both variable domains and constant regions thus their construction and ultimate expression is somewhat more complex. Fusion Antibodies are experts with over 15 years experience in antibody production. If a monoclonal antibody is available the fab fragment can be created enzymatically, or by first sequencing the variable domains followed by recombinant expression. It is also possible to create a fab fragment whenever there is no monoclonal antibody available simply from the antibody variable region sequences if known.

For more information contact us today! Email or call UK +44 (0)28 9043 2800

IgG class switching

Fusion Antibodies offer antibody isotype switching as a service. Using our own technology and in-house expertise we are able to change any antibody isotype from any species to any other IgG isotype. The antibody isotype switching process involves replacing only the constant region of the antibody while maintaining the variable regions and therefore specificity of your antibody. Antibody class switching does not affect antigen specificity, retains antigen affinity and allows interaction with different effector molecules.

Antibody class switching can benefit your research by giving you control over the level of immune cell engagement, avidity and half-life of your antibodies. Fusion Antibodies have the expertise and experience to provide advice and guidance throughout each stage of your customized, mile-stoned antibody engineering project.

The main class switches that Fusion Antibodies offers are listed below however we are able to change an antibody of any isotype from any species to any other IgG isotype. Simply provide us with two standard freeze down vials of your antibody producing cell line and then inform us of the desired isotype and Fusion Antibodies will sequence the antibody, class switch to the required isotype and (if necessary) express your new antibody.

Your Antibody

Fusion Antibodies

Class Switching Service

Resulting Isotype
Human IgA Human IgG1
Human IgD Human IgG2
Human IgE Human IgG3
Human IgG Human IgG4
Human IgM Murine IgG1-4
All Murine Antibodies


For more information contact us today! Email or call UK +44 (0)28 9043 2800

Bi-specific antibody production

Fusion Antibodies are proud to offer a new and unique service of generating antibodies which are hybrids of two unique antibody molecules. With the recent press attention surrounding the first FDA approved bi-specific antibody; catumaxomab and the announcement of the humanization of Fusion Therapeutics very own Fsn1006 dual-targeting antibody, there has never been a better time to test the combined effects of your antibodies.

[image of bi-specific antibodies]

We can aid in the design of your bi-specific antibody and all we need to start would be freeze down vials of your antibody expressing cell lines. Fusion Antibodies will then sequence your antibodies, combine them in an engineered format and (if necessary) express your new antibody.

For more information contact us today! Email or call UK +44 (0)28 9043 2800

Other engineered antibody formats

In addition to the above antibody fragments, Fusion Antibodies can also design a number of other formats including:

  • Diabody
  • Minibody
  • Triabody

Contact Us

We would like to keep you informed by email. Are you happy to receive information from us?

Related Services

Make an Enquiry

Feel free to get in touch for any further information. We would love to hear from you.

Contact Us