Antibody Engineering Services

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Fusion Antibodies offers a range of antibody engineering services including chimeric, scFv, Fab, F(ab')2, bi-specific and more!

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We are experts at antibody engineering and can produce a range of custom antibodies

We offer a range of custom antibody configurations which are sure to meet your requirements. We can produce antibodies in a wide range of formats including scFv, Fab, chimerics and many more. We can also combine our antibody engineering services with our humanization services and express your humanized antibodies in an alternative format. There is no other company in the world that has the same experience in engineered antibody production. With antibody engineering services built on a constant development cycle since 2001, we have unrivalled, industry-leading knowledge and expertise which you can take advantage of for your next antibody engineering project.

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Contact us about Antibody Engineering Services


Popular engineered antibody configurations

Fusion Antibodies is a CRO which offers a range of Antibody Engineering services. Some of our most popular antibody configurations are shown below but many more are possible. If you have an idea for an alternative antibody construct contact us on the form above and we would be happy to discuss!

Chimeric Antibodies

Our antibody chimeric antibody engineering service allows you to rapidly convert the species isotype of your antibody to validate its potential in various therapeutic or diagnostic assays. Variable domains sequenced from your hybridoma cell line can be recombined with constant domains of you choice.

  • Reduce the immunogenicity of the antibody in vivo.
  • Many constant domains available
  • Optional Antibody Sequencing service available
  • Compare effector functions
  • Optimize allotype

scFv antibody fragments

There are many advantages in using scFv’s, particularly in the fields of therapeutics and diagnostics. scFv’s offer several advantages over monoclonal antibodies as carriers of radionuclei and drugs to tumors, including greater tumour penetration due their small size, low kidney uptake, rapid blood clearance and a lower negative response by the human immune system.

Fab and F(ab')2 antibody fragments

Fab fragments differ from scFv’s as they contain both variable domains and constant regions thus their construction and ultimate expression is somewhat more complex. If a monoclonal antibody is available the fab fragment can be created enzymatically, or by first sequencing the variable domains followed by recombinant expression. It is also possible to create a fab fragment whenever there is no monoclonal antibody available simply from the antibody variable region sequences if known.

For more information contact us on the form below, email or call UK +44 (0)28 9043 2800

IgG class switching

Fusion Antibodies offer antibody isotype switching as a service. Using our own technology and in-house expertise we are able to change any antibody isotype from any species to any other IgG isotype. The antibody isotype switching process involves replacing only the constant region of the antibody while maintaining the variable regions and therefore specificity of your antibody. Antibody class switching does not affect antigen specificity, retains antigen affinity and allows interaction with different effector molecules.

Antibody class switching can benefit your research by giving you control over the level of immune cell engagement, avidity and half-life of your antibodies. We have the expertise and experience to provide advice and guidance throughout each stage of your customized, mile-stoned antibody engineering project.

The main class switches which we offer are listed below however we are able to change an antibody of any isotype from any species to any other IgG isotype. Simply provide us with two standard freeze down vials of your antibody producing cell line and then inform us of the desired isotype and Fusion Antibodies will sequence the antibody, class switch to the required isotype and (if necessary) express your new antibody.

Your Antibody

Class Switching Service

Resulting Isotype
Human IgA Human IgG1
Human IgD Human IgG2
Human IgE Human IgG3
Human IgG Human IgG4
Human IgM Murine IgG1-4
All Murine Antibodies


Bi-specific antibody production

Fusion Antibodies are proud to offer a new and unique service of generating antibodies which are hybrids of two (or more) unique antibody molecules. With the recent press attention surrounding the first FDA approved bi-specific antibody; and the announcement of the humanization of Fusion Therapeutics very own Fsn1006 dual-targeting antibody, there has never been a better time to test the combined effects of your antibodies. We can aid in the design of your bi-specific antibody and all we need to start would be freeze down vials of your antibody expressing cell lines. Fusion Antibodies will then sequence your antibodies, combine them in an engineered format and (if necessary) express your new antibody.

Other Antibody Engineering Services

In addition to the above antibody configurations, we can also design a number of other formats including:

  • Diabody
  • Minibody
  • Triabody

For more information contact us on the form below, email or call UK +44 (0)28 9043 2800

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