Gene-to-protein expression

Gene-to-protein expression

Our gene-to-protein expression services are your first choice for high quality and reliable protein production in either mammalian or bacterial systems.  

Whether to underpin a protein engineering campaign, to support antigen production or for batch production of your therapeutic candidate, our goal is to deliver exceptional proteins.

We have extensive expertise and knowledge when it comes to protein expression in both mammalian and bacterial systems. Reliable and high quality protein production underpins any preclinical development program and is essential for a robust data package and downstream success.

We have a dedicated team of biochemists that understand the challenges of protein expression, purification and characterisation. Furthermore our protein team work closely with our sequence engineers to ensure we achieve the best possible sequence for optimal production of your protein.

Fusion’s protein expression service is designed to provide you reliable recombinant proteins with optimal yields in rapid timelines.

 

Mammalian Expression 

Mammalian expression is recommended when you require functional recombinant proteins with post-translational modifications. Our mammalian expression service gives you early access to your proteins expressed in CHO or HEK cells without the lengthy time-scales of cell line development. Transient expression in CHO cells is the workhorse of any therapeutic protein program and we aim to deliver the best possible service to underpin your preclinical development program.

Bacterial Expression

Bacterial expression systems remain a good option for production of small proteins and antibody fragments and we at Fusion are experienced in the production of these molecule types. We offer expression optimisation in a range of bacterial strains, combined with protein refolding (if necessary) and optimised purification strategies.

Both our transient mammalian and bacterial expression services are cost-effective solutions, which allow you to reliably scale up from 50ml to 1L scale. All expression projects are supported by a range of purification and analytical strategies, which can be tailored to your specific molecule.

Protein Purification

Every molecule is unique and we understand that tailored purification strategies may be required for every single project. We offer a range of purification techniques that can be adopted for your specific molecule for robust and scalable batch production delivering proteins of high  purity and low endotoxin levels. These methods can be established for batch release and consistent QC.

Scalability is important for effective downstream development of your molecule and we ensure that methods are established with this in mind.  Whether in the native format or tagged, we have multiple purification options including Protein A/G affinity chromatography, IMAC, IEC, SEC, HIC. We can also perform high resolution chromatography for analysis of your protein and for efficient purification at the small-scale level.

We will approach your project with flexibility and with the end in mind.

Next steps

Fusion Antibodies is an experienced partner in developing stable cell lines for research and manufacturing purposes. We offer a range of cell line development options which are flexible and adaptable to meet the specific requirements of your project. To find out more visit our cell line development page.

Contact us today