Affinity Maturation

Affinity Maturation

We go further than Affinity Improvement. 

Rational Affinity Maturation Platform; RAMP™

Our Affinity Maturation service, Rational Affinity Maturation Platform (RAMP™), will give you the assurances you need to confirm that you are moving forward with the best possible antibody.

RAMP™ adopts lessons from nature and combines a rational approach to library design, with rapid in silico selection for both improved binding potential and predicted stability. The result is that we will generate an enriched ‘micro-library’ of variants with improved stability and affinity for the target.

We go the extra mile to deliver exceptional antibodies by applying our expert knowledge and cutting edge technologies. Our RAMP™ platform is designed to accelerate your lead selection program, providing you with the best chances of success in the clinic.

Affinity Maturation

Rational Library Design

Fusion Antibodies have developed a novel, proprietary, method for library design, which explores the natural somatic hypermutation space of your antibody sequence. Using this approach, substitutions are incorporated into both the framework and CDR regions and restrictions ensure that safety or manufacturing liabilities will not be introduced. This unique approach can allow for natural mutations that may unexpectedly improve the profile of your antibody.

In Silico Selection

Using in silico modelling and molecular docking we interrogate the antibody library, selecting those with predicted improvements in both binding potential and stability. Balance of function and developability is a key principle of RAMP™.

This rapid selection approach enables us to focus a library of >1015 variants down to a micro-library of approx. 100 sequences, for in vitro expression and characterization.

Micro Library Expression and Characterisation

An important benefit of our approach to Affinity Maturation is that there is no need to express a large physical library of variants. With RAMP™, we express a micro-library of selected variants in CHO cells in the IgG format. There is no bias for scFv or Fab format and no bias for stability in bacterial expression. Binding kinetics and biophysical attributes of all variants are characterized in vitro.

A high proportion of expressed mAbs display improved affinity compared to the parental mAb; offering a rich pool for selection of your lead candidate antibody or start point for repeated rounds of RAMP™.

Highlights from our approach to Affinity Maturation – RAMP™

  • Natural approach to library design
  • No need to generate a large physical library of variants
  • No bias for scFv format or bacterial expression (required for phage display)
  • Characterisation of mAbs in full IgG format
  • Rapid timelines (under 3 months)
  • High proportion of expressed mAbs display improved affinity
  • >10-fold affinity improvement per round

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