Protein synthesis and purification are offered as a unique service by Fusion Antibodies. The techniques developed by Fusion are high-throughput methods that enable the rapid production of purified fusion proteins. The core of our methodology involves the rapid production of proteins from genes sequences and to facilitate these processes we can clone the gene of interest into a number of proprietary vector systems – which express multiple fusions to rapidly assess solubility of the protein. Once the soluble fusion construct has been identified, we are able to rapidly purify the protein.

The fusion proteins are purified using low pressure chromatography systems based on Amersham's AKTAPrime. The fusion proteins contain tags that are amenable to purification by metal ion chromatography. All proteins that contain a fusion tag bind to the column and are retained, the other proteins wash through the column to waste. The bound proteins are then removed from the column and collected as fractions. Fractions containing protein are analysed by SDS/PAGE to determine size and purity. Western blots are also carried out on the samples to confirm that the proteins are the correct size and are not truncated. This results in proteins with a purity of over 95%. 

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